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slow type myhc  (Novus Biologicals)


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    Novus Biologicals slow type myhc
    Slow Type Myhc, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/skeletal+muscle+myhc+slow+antibody/Slow+Skeletal+Muscle+Myosin+Heavy+Chain+Antibody+(96J)/pmc12914295-87-18-22
    Average 94 stars, based on 5 article reviews
    slow type myhc - by Bioz Stars, 2026-10
    94/100 stars

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    Expressing:

    Article Title: Histamine is a molecular transducer of adaptation to endurance exercise training in humans
    Article Snippet: .. A total of 4 primary antibodies were used to determine immunoreactivity of myosin heavy chain (MyHC) isoforms expressed by different muscle fiber types: (1) A specific skeletal muscle MyHC Slow antibody (Novus, NBP2-50299 Mouse IgG, Centennial, CO, USA) was used at a 1:10 dilution to identify slow type I muscle fibers. (2) A specific fast skeletal muscle MyHC 2A antibody (SC-71 Developmental Studies Hybridoma Bank, Mouse IgG, Iowa City, IA, USA) was used at a 1:1 dilution to identify fast type IIa muscle fibers. (3) A specific skeletal muscle MyHC 2X antibody (Novus, NBP1-22811 Mouse IgG, Centennial, CO, USA) was used at a 1:10 dilution to identify fast type IIx muscle fibers. (4) A non-specific skeletal muscle MyHC Fast antibody (Vector VP-M665, Mouse IgG, Burlingame, CA, USA) was used at a 1:10 dilution to identify all fast type II muscle fibers and by exclusion any fibers expressing MyHC 2B (type IIb muscle fibers for which there is no specific antibody). .. After the primary antibodies incubated overnight, the slides were washed thoroughly in 0.1 M phosphate buffer and then all sections were incubated for 2 h in secondary antibody at a 1:200 dilution (Cy3 donkey anti-mouse IgG, ThermoFisher Scientific, Waltham, MA, USA) to assist in detection during imaging.



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